Wednesday, September 15, 2010

Diagnosis Virology of Avian Influenza

Diagnosis of virology conducted to ensure whether the infecting subtype H5N1 or another. How ataumetode examination used to detect avian influenza virus subtype H5N1 were:
1. Serological examination to detect antibody / antigen on the patient's body
2. Isolation and identification of virus
3. Detecting H5 spesifih RNA by RT-PCR (Reverse Transcriptase Polymerase Chain Reaction)

A. Serology Examination
Antobodi will arise because of the efforts of the body to protect yourself from viruses that enter. Several methods can be used to detect influenza A virus-specific antibodies are formed in the body is the hemagglutination inhibition test, complement fixation test and enzyme-linked immunosorbent assay (ELISA). Antigens in patients with influenza A virus infection can be examined with ELISA method.

Materials used for serological examination from the patient's blood serum. Currently available rapid test in the form of kits that can quickly deliver results, but less accurate. Results will be received within approximately 1-2 hours.

Because the results are less accurate and can not find a subtype of influenza A virus that infects, it must perform the examination began a more sensitive and precise to ensure that the H5N1 virus as the cause.

B. Isolation and Identification of Viruses
How to inspection by isolating the virus is the most excellent and very decisive. However, this examination requires sophisticated tools and techniques are also experts. So it is not used routinely in the laboratory.

Examination materials which can be used for the isolation of the virus is grown from sputum in patients with pneumonia. Virus isolation is done by planting material that will be checked on a network or embryonated chicken eggs, then examined influenza A virus subtype that has isolated them.

C. RT-PCR (Reverse Transcriptase Polymerase Chain Reaction)
Method of examination was conducted to provide accurate results about the cause of the H5N1 avian influenza has been done and as a serologic confirmation. There are several kinds of methods have been developed to detect viral RNA of influenza A.

Molecular diagnosis has several advantages, such as sensitive. Because it can detect small amounts of viral components, is able to distinguish the genetic material with a higher level of differentiation, providing results in a relatively more rapid.

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